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KMID : 0380020070220020067
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 2 p.67 ~ p.72
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Research Papers : In Vitro Study of Fluorescence Detection for Protoporphyrin IX Induced from 5-Aminolevulinic Acid in Incubated Lung Cancer Cells
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Kim Myung-Hwa
Lee In-Seon
Lee Chang-Seop
Kim Kyung-Chan
Kim Hyun-Jeong
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Abstract
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This study investigates the optimal method of administrating 5-aminolevulinic acid (5-ALA) in the context of fluorescence detection by analyzing protoporphyrin IX (PpIX) fluorescence in the cultured normal and cancer cells. 5-ALA was injected as a photosensitizer to the lung cancer cells (A549, NCl-H460) and normal lung cells (Hel299). Hel299, A549, and NCl-H460 cells were incubated with various concentrations of 5-ALA (0~800 μg/mL). The accumulation of PpIX induced by 5-ALA was observed in A549, NCl-H460 and Hel299 cells. The cell viability was estimated by means of the MTT assay. Formation of PpIX was measured by fluorescence spectroscopy. Especially, formation of PpIX in cancer cells was higher than normal cells. This study suggests that the difference of PpIX induced in normal and cancer cells treated with 5-ALA may use by means of fluorescence diagnosis for cancer.
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KEYWORD
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5-aminolevulinic acid, protoporphyrin IX, photosensitizer
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